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Int J Drug Res Clin. 2024;2: e19.
doi: 10.34172/ijdrc.2024.e19
  Abstract View: 69
  PDF Download: 82

Original Article

Combined Blockade of Interleukin-6 and β-Catenin Inhibits Breast and Colon Tumor Growth In Vitro

Maryam Farajzadeh 1 ORCID logo, Mohammad-Salar Hosseini 2, Farzaneh Eshaghi 1, Samira Mohebbi 3, Mohammad Hojjat-Farsangi 4, Farhad Jadidi-Niaragh 1,2,5* ORCID logo

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Research Center for Integrative Medicine in Aging, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran
3 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Oncology-Pathology, Karolinska Institute, Bioclinicum, Stockholm, Sweden
5 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Farhad Jadidi-Niaragh, Email: jadidif@tbzmed.ac.ir

Abstract

Background: As the primary regulator of tumor activities, the tumor microenvironment contains several factors contributing to tumor progression and metastasis. Interleukin 6 (IL-6) and β-catenin are two key factors in the tumor microenvironment, which are effective in tumor progression in primary studies. This study aimed to evaluate the impact of IL-6 and β-catenin blockade on tumor growth and progression in vitro.

Methods: In this study, small interfering RNA (siRNA) technology was used to target IL-6 and β-catenin using two murine cell lines, 4T1 (breast cancer) and CT26 (colon cancer). The target gene expression was analyzed by applying quantitative real-time polymerase chain reaction assays. The MTT assay was used to measure how a treatment could affect the cells’ survival ability. All data were analyzed by GraphPad Prism 9, and P-values less than 0.05 were considered significant.

Results: Both cell lines demonstrated downregulation in the expression patterns of genes involved in apoptosis, metastasis, proliferation, and angiogenesis. Both cell lines showed significantly decreased IL-6 secretion following IL-6 targeting. Moreover, simultaneous targeting of IL-6 and β-catenin factors significantly reduced the survival of cancer cells.

Conclusion: These results revealed that targeting β-catenin and IL-6 simultaneously may be a useful treatment strategy for colon and breast cancers. Further investigations are required to determine the effectiveness of these interventions and adjust their safety.


Please cite this article as follows: Farajzadeh M, Hosseini MS, Eshaghi F, Mohebbi S, Hojjat-Farsangi M, Jadidi-Niaragh F. Combined blockade of interleukin-6 and β-catenin inhibits breast and colon tumor growth in vitro. Int J Drug Res Clin. 2024; 2: e19. doi: 10.34172/ijdrc.2024.e19
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Submitted: 10 Aug 2024
Accepted: 11 Sep 2024
ePublished: 17 Nov 2024
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